Scophthalmus maximus L. larviculture
نویسندگان
چکیده
The aerobic bacterial flora in the gut of turbot larvae and their influence on larval survival was examined. Two turbot experiments were run with six replicates each time. Large variation, from 0% up to 44%, was observed in the survival percentage of turbot larvae. There was no correlation between the number of bacteria present in the gut of turbot larvae and the larval survival rate. During both experiments, all replicates followed nearly the same rate of bacterial development in the gut of turbot larvae going from circa 10 CFU larva just before first feeding at day 3 post hatch to 10 CFU larva at day 9 post hatch. In total, 127 bacterial isolates from 12 rearing tanks were sampled for further investigation. Based on their fatty acid profile obtained by FAME-analysis, and using principal component Ž analysis, the isolates were subdivided in 12 major gaschromatographic-groups or clusters GC. Ž . groups , 11 isolates remained unclustered. Four specific GC-groups namely cluster A, B, I and J were selected as potential beneficial bacteria for turbot larviculture as the majority of the isolates of these clusters derived from rearing tanks with a survival percentage higher than 35%. Representative isolates of these clusters were screened on their ability to enhance the survival rate as well as the poor reproducibility in larval survival in a small-scale turbot confrontation test. Also, a Vibrio mediterranei Q40 strain, isolated from sea bream larvae, was included in these small-scale confrontation tests. Only cluster A and the V. mediterranei Q40 strain had a distinct positive and reproducible effect on larval survival. In conclusion, cluster A and V. mediterranei ) Corresponding author. Tel.: q32-9-264-3754; fax: q32-9-264-4193. Ž . E-mail address: [email protected] L. Huys . 0044-8486r01r$ see front matter q2001 Elsevier Science B.V. All rights reserved. Ž . PII: S0044-8486 00 00474-9 ( ) L. Huys et al.rAquaculture 193 2001 25–37 26 Q40 seemed to play a role as first coloniser of the gut of turbot larvae and could prevent the colonisation of the gut by opportunistic bacteria. q 2001 Elsevier Science B.V. All rights reserved.
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تاریخ انتشار 2000